Fatores associados a medidas biométricas fetais pequenas para idade gestacional no Estudo MINA-Brasil / Factors associated with small-for-gestational-age fetal biometric measurements in the MINA-Brazil Study

Introdução - As elevadas taxas de mortalidade neonatal e a prevalência de bebês nascidos pequenos para idade gestacional (PIG) ainda apresentadas por regiões de baixa e média renda indicam a necessidade de investigação sobre fatores que influenciam o crescimento fetal. Medidas biométricas fetais

Introduction Elevated rates of neonatal mortality and small-for-gestational-age (SGA) babies in low- and middle-income regions indicate the need to investigate factors associated with fetal growth. Fetal biometric measurements below the 10th percentile for gestational age (GA) reflect failure in achieving growth potential and provide opportunities for prenatal interventions. Objective To investigate factors associated with fetal biometric measurements below the 10th percentile assessed at early third trimester among pregnant women in the MINA-Brazil study. Methods This was a prospective analysis of pregnant women living in the urban area of Cruzeiro do Sul (AC), followed up since the antenatal period. Screening of participants took place from February 2015 to January 2016. A sociodemographic and health history interview was carried out along with two clinical assessments, scheduled between the second and third trimesters of pregnancy, to collect data on lifestyle factors and complications during pregnancy, anthropometric evaluation, blood collection, and ultrasound scan assessing fetal biometric measurements of head circumference (HC), abdominal circumference (AC) and femoral length (FL). Poisson regression models with hierarchical selection of variables were fitted for factors associated with occurrence of fetal measurements below the 10th percentile at early third trimester. Prevalence ratios (PR) and 95% confidence intervals (95%CI) were estimated. Results Among 426 participants (mean age 25 years (SD 6.4) and gestational age 27.8 weeks (SD 1.7)), 11.3%, 8.9% e 9.4% had fetuses with HC, AC, FL below the 10th percentile at early third trimester. Fetal biometric measurements below the 10th percentile were negatively associated with higher maternal education level, which denoted over 50% of protection (HC: PR 0.47, 95%CI 0.28; 0.81; AC: PR 0.48, 95%CI 0.26; 0.87; and FL: PR 0.48, 95%CI 0.27; 0.86). Adjusted for maternal education level, nulliparity (PR 1.94, 95%CI 1.10; 3.43), higher pre-gestational body mass index (BMI) (PR 1.06, 95%CI 1.01; 1.11), and pre-gestational alcohol consumption (PR 1.80, 95%CI 0.98; 3.30) were associated with HC measurements below the 10th percentile. Maternal height (p for trend 0.039) and pre-gestational alcohol consumption (PR 2.55, 95%CI 1.31; 4.96) were also associated with AC measurements below the 10th percentile. Association between higher average screen time per day during antenatal follow-up and FL measurements below the 10th percentile were observed as well (p for trend 0.031). Higher maternal education level, which may provide better material and non-material conditions, seems to protect fetal growth from failure to reach potential fetal size for GA. Positive associations observed with obstetric and antenatal conditions may be mediated by biological factors or gestational dysfunctions to incur in fetal measurements below the 10th percentile. Conclusion The occurrence of fetal biometric measurements below the 10th percentile for GA and their associated factors corroborate pre-pregnancy and antenatal care improvements anchored in equity policies, and for new strategies prior to birth that optimize the window of opportunity in the first thousand days of life.

Rabbit growth plate morphology in temporary bilateral blocking / Morfología de la placa de crecimiento de conejos durante bloqueo bilateral temporario

Blocking of the growth plate (GP) using plates with screws (tension band plating) is a modern method used to correct deformities and moderate leg length discrepancy in growing children. Determining the duration of temporary bilateral blocking without the occurrence of irreversible changes of GP is of paramount importance important. Methods: Two-month-old Californian breed male rabbits (n=30) were exposed to bilateral blocking of the distal GP of the right femur locking plates with screws for 3, 5, and 7 weeks. The fixators were removed after 5 and 7 weeks in 18 rabbits and 3 weeks after that, animals were sacri!ced. The contralateral limb was used as a control. Histological, histomorphometric, and X-ray analyses were performed. Results: During GP blocking, its height gradually decreased. This decreased was more pronounced after 7 weeks. Destructive changes progressed with an increase in the blocking duration. Three weeks after discontinuation of the bilateral blocking that lasted 5 weeks, the height of the GP signi!cantly increased 1.2 times on the lateral side and 1.9 times on the medial side (p<0.001) compared to the control. When blocking was discontinued after 7 weeks, the structure of the GP was partially restored after 3 weeks, the height of GP signi!cantly increased 1.2 times on the lateral side, and 1.07 times on the medial side (p<0.01) compared to the control. Conclusion: Restoration of the structuralfunctional features of the GP after the removal of the plates depends on the duration of temporary bilateral blocking, which must be taken into account in the clinical setting. (AU)

El bloqueo de la placa de crecimiento (PC) utilizando placas con tornillos (banda de tensión) es un método moderno utilizado para corregir deformidades y alteraciones moderadas en la longitud de las piernas en niños en crecimiento. Es de suma importancia determinar cuál debe ser la duración del bloqueo bilateral temporal sin que ocurran cambios irreversibles en la PC. Métodos: Conejos machos de raza californiana de dos meses de edad (n = 30) fueron expuestos al bloqueo bilateral de la PC distal colocando placas del fémur derecho con tornillos durante 3, 5 y 7 semanas. Los fijadores fueron retirados después de 5 y 7 semanas en 18 de los conejos, y 3 semanas después los animales fueron sacrificados. La extremidad contralateral se utilizó como control. Se realizaron análisis histológicos, histomorfométricos y de rayos X. Resultados: Durante el bloqueo de la PC, su altura disminuyó gradualmente. Esta disminución fue más pronunciada después de 7 semanas. Los cambios destructivos se incrementaron a medida aumentaba la duración del bloqueo. Tres semanas después de la interrupción del bloqueo bilateral que duró 5 semanas, la altura de la PC aumentó significativamente 1.2 veces en el lado lateral y 1.9 veces en el lado medial (p <0.001) en comparación con el control. Conclusión: La restauración de las características funcionales estructurales de la PC después de la extracción de las placas depende de la duración del bloqueo bilateral temporal, lo que debería tenerse en cuenta en el tratamiento clínico de estas alteraciones. (AU)

Maternal malnutrition diet during lactation period leads to incomplete catch-up growth in femur of the pups at adulthood / Restricción alimentaria materna durante la lactancia conduce a la recuperación del crecimiento incompleto en el fémur de las crías en la edad adulta

The aim of this study was to evaluate the effects of maternal protein and energy restriction during lactation, analyzing on morphological dimensions whether there was catch-up growth through normative nutrition restored, as well as on mechanical axis of femur of the offspring at adulthood. At parturition, Wistar rat dams were randomly assigned to the following groups: 1) control group (C) - free access to a standard laboratory diet containing 23 % protein, 2) protein-energy restricted group (PER) - free access to an isoenergetic, protein-restricted diet containing 8 % protein, and 3) energy-restricted group (ER) ­ fed with restricted amounts of a standard laboratory diet. At weaning, all pups were separated of dams and received free access to a standard laboratory diet containing 23 % protein until 180 days, when the rats were anesthetized and sacrificed. The dimensions of excised pup femur were measured directly using pre-established anatomical points. Morphometric analysis of the femur (macroscopic) showed that most of the measurements in the ER and PER groups were significantly lower than in the control group, with the greatest reductions occurring in the PER group and several structural abnormalities. Our results show that protein and energy restriction during lactation leads to an incomplete catch-up growth in adulthood. The femur showed significant reduction in most of the parameters in the two treated groups, particularly the PER group, when compared to the control group.

El objetivo de este estudio fue evaluar los efectos de la restricción de la proteína materna y de energía durante la lactancia, y analizar las dimensiones morfológicas si hubo recuperación del crecimiento a través de la nutrición normativa restaurada, así como en el eje mecánico del fémur de la descendencia en la edad adulta. En el parto, las crías de ratas Wistar fueron agrupadas aleatoriamente en los siguientes grupos: 1) control (C) - con acceso libre a una dieta estándar del laboratorio, que contenía 23 % de proteínas; 2) con restricción de proteínas y energía (PER) - acceso libre a una dieta isoenergética, con restricción de proteínas, conteniendo un 8 % de éstas y 3) grupo con restricción de energía, alimentado con restricción en la cantidad de alimento de la dieta estándar del laboratorio (ER). Al destete, todas las crías fueron separadas y recibieron las dietas establecidas hasta los 180 días (d180), luego fueron anestesiadas y sacrificadas. Las mediciones de los fémures de las crías de rata fueron obtenidas por puntos anatómicos preestablecidos. El análisis morfométrico de fémur demostró que la mayoría de las mediciones en los grupos ER y PER eran significativamente menores que las del grupo control, con mayores reducciones en el grupo PER. Nuestros resultados muestran que las restricciones de energía y proteínas durante la lactancia conducen a una recuperación del crecimiento incompleto en la edad adulta. El fémur mostró una reducción significativa en la mayoría de los parámetros en los dos grupos tratados, en particular el grupo de PER, en comparación con el grupo control.

Inhibición del crecimiento longitudinal en hemiepifisiodesis: análisis experimental en conejos / Longitudinal growth inhibition in hemiepiphysiodesis: experimental analysis in rabbits

Introducción: La modificación del eje de un miembro mediante la detención parcial del crecimiento (hemiepifisiodesis), es un procedimiento habitual en la cirugía ortopédica pediátrica. Sin embargo no está bien establecido cuánto afecta este procedimiento al crecimiento final del hueso. El objetivo del presente trabajo fue determinar en forma experimental la inhibición del crecimiento longitudinal producido por una hemiepifisiodesis. Materiales y métodos: Se utilizaron, 10 conejos de 8 semanas de vida, a los que se les colocó una grapa en el fémur distal externo derecho. En la 8ª semana del estudio (16 semanas de vida) se realizó la comparación radiológica e histológica de ambos fémures. Resultados: La longitud final media de los fémures con grapas a las 8 semanas finalizado el experimento fue de 89,6mm (rango 87mm-95mm) versus 95,7mm (rango 91mm-100mm) de los fémures sanos. El porcentaje de inhibición media del crecimiento fisario al final del experimento fue de 6,58% (rango 3.2%-10%). Conclusiones: Se demostró experimentalmente la disminución global de la actividad de la fisis durante la hemiepifisiodesis. Esto debe ser tenido en cuenta cuando indicamos una hemiepifisiodesis unilateral en un niño con una discrepancia previa de longitud.

Bone development trend in the knee joint of Tibetan teenagers in Aba Prefecture of Sichuan Province / 法医学杂志

OBJECTIVE@#To discuss bone development trend in the knee joint of Tibetan teenagers in Sichuan province and to effectively update the database for estimating the living age of Tibetan teenagers in terms of bone age of the knee joint.@*METHODS@#Radiographs including epiphysis of distal femur, proximal tibia and proximal fibula were taken from 483 Tibetan male and female teenagers aged from 14 to 19 years old in Aba prefecture of Sichuan province in order to observe epiphyseal growth situation. The descriptive data of the epiphyseal closure ages of these teenagers' knee joints were statistically analyzed by SPSS 16.0 software.@*RESULTS@#The distal femur epiphyseal closure occurred earliest, while the proximal fibula epiphyseal closure occurred latest. The epiphyseal growth of knee joints of females occurred about one year earlier than that of males.@*CONCLUSION@#The forensic information and data related to Tibetan teenagers' bone age identification should be updated regularly. These results provide potential value for the practice of forensic medicine, anthropology and clinical medicine.

Low-level laser on femoral growth plate in rats / Laser terapêutico de baixa potência na placa de crescimento de ratos

PURPOSE: To determine the influence of low-level laser therapy on femoral growth plate in rats. METHODS: Thirty male Wistar rats aged 40 days were divided into two groups, G1 and G2. In G1 the area of the distal growth plate of the right femur was irradiated at one point using GaAlAs laser 830 nm wavelength, output power of 40 mW, at an energy density of 10 J/cm². The irradiation was performed daily for a maximum of 21 days. The same procedure was done in G2, but the probe was turned off. Five animals in each group were euthanized on days 7, 14 and 21 and submitted to histomorphometric analysis. RESULTS: In both groups the growth plate was radiographically visible at all moments from both craniocaudal and mediolateral views. On the 21st day percentage of femoral longitudinal length was higher in G2 than G1 compared to basal value while hypertrophic zone chondrocyte numbers were higher in G1 than G2. Calcified cartilage zone was greater in G1 than in G2 at all evaluation moments. Angiogenesis was higher in G1 than in G2 at 14th and 21st days. CONCLUSION: The low-level laser therapy negatively influenced the distal femoral growth plate.

OBJETIVO: Determinar a influência do Laser Terapêutico de Baixa Potência sobre a placa de crescimento de ratos. MÉTODOS: Trinta ratos Wistar machos com 40 dias de idade foram divididos em dois grupos, G1 e G2. O grupo G1 foi submetido à irradiação com laser GaAlAs 830 nm, potência de saída de 40 mW, e densidade de energia de 10 J/cm2. A irradiação foi aplicada diariamente por um período máximo de 21 dias. O mesmo procedimento foi realizado no grupo G2, com a probe desativada. Cinco animais em cada grupo foram sacrificados nos dias 7, 14 e 21 e submetidas à análise histomorfométrica. RESULTADOS: Em ambos os grupos, o disco fisário esteve radiograficamente visível em todos os momentos nas incidências craniocaudal e médio-lateral. No 21º dia a porcentagem de comprimento longitudinal do fêmur foi maior em G1 que em G2 em relação ao valor basal, e o número de condrócitos da zona hipertrófica foi maior em G1 que em G2. A zona de cartilagem calcificada estava maior em G1 em relação a G2 em todos os momentos de avaliação. A angiogênese foi maior em G1 que em G2 nos 14º e 21º dias. CONCLUSÃO: A terapia com laser terapêutico de baixa potência influenciou negativamente o disco fisário distal do fêmur de ratos.

Impacto da dieta hiperlipídica contendo óleo de canola ou de soja no desenvolvimento da adiposidade abdominal e estrutura óssea / Impact of high-fat diet containing canola oil or soybean in the development of abdominal adiposity and bone structure

A baixa relação de ômega-6/ômega-3 esta relacionada com propriedades benéficas para a saúde óssea. No entanto, a dieta rica nestes compostos pode levar a obesidade. Adipócitos e osteoblastos derivam de células progenitoras comuns, e o consumo de óleo de canola pode ter ação adipogênica e osteogênica. Nosso objetivo foi avaliar a adiposidade abdominal, insulina e estrutura óssea em ratos tratados com dieta contendo baixa relação ômega-6/ômega-3, proveniente do óleo de canola. Após desmame, os ratos foram divididos em grupos alimentados com dieta normocalórica: Controle (S) e experimental (C), contendo 7m1/100g de óleo de soja ou de canola e grupos tratados com dieta rica em lipídios: Controle (7S) ou hiperlipídico contendo 19m1/100g de óleo de soja (19S) ou de canola (19C), até completarem 60 dias de idade. Os dados foram significativos com P<0,05. No primeiro modelo, o grupo C apresentou redução de: Massa e área do adipócito intra-abdominal; Colesterol; Insulina; Densidade mineral (DMO) e massa óssea total e na coluna vertebral; Massa do fêmur; Espessura da diáfise; DMO do fêmur e das vértebras lombares e radiodensidade da cabeça do fêmur. No segundo modelo, os grupos 19S e 19C apresentaram maior ingestão calórica, densidade corporal, massa de gordura intra-abdominal, e maior massa e comprimento do fêmur e da coluna lombar. O grupo 19S apresentou maior área e menor número de adipócitos da região retroperitoneal. Glicose e a insulina foram aumentadas no grupo 19C vs. 7S. A tomografia do fêmur revelou maior radiodensidade na região proximal e da coluna lombar, no grupo 19C. Sugerimos que a quantidade e o tipo de lipídio consumido, após o desmame, induzem não somente o desenvolvimento corporal e os depósitos de gordura, além de afetarem a resistência insulínica e a saúde óssea

The lower ratio of omega-6 to omega-3 polyunsaturated fatty acids is associated with healthy bone properties. However, fat diets can induce obesity. Adipocytes and osteoblasts derive from a common progenitor, and canola oil intake may have an adipogenic and osteogenic effect. Our objective was to evaluate the intra-abdominal adiposity, insulin and bone growth in rats fed diet containing lower ratio of omega-6 to omega-3, provided in canola oil. After weaning, rats were divided into groups fed with normocaloric diet: control (S) and experimental (C), containing 7 m1/100g soybean or canola oil, respectively and groups fed with fat diet: control (7S) or fat diets containing 19 m1/100g soybean oil (19S) or canola oil (19C), until they 60 days old. Differences were considered significant with P<0,05. In normocaloric diet model, C group showed a significant reduction in: Intra-abdominal fat mass; Area of adipocyte; Cholesterol; Insulin; Total body and spine bone mineral content and bone area; Femur mass; Width of the diaphysis; Femur and lumbar vertebrae bone mineral density and radiodensity of femoral head. To high-fat diet model, 19S and 19C groups showed higher energy intake, body density growth, intra-abdominal fat mass and higher femur mass and, lumbar vertebrae mass and length. 19S showed higher area and lower number of retroperitoneal adipocytes. Glucose and insulin were significantly increased in 19C compared to 7S group. Computed tomography of femur revealed higher radiodensity in proximal femoral epiphysis and lumbar vertebrae of 19C. We suggest that the amount and the source of fat used in the diet, after weaning, induce not only the body and fat depots growth, besides affecting the insulin resistance and the bone health

Evaluación de las variaciones del ángulo de torsión del fémur en fémures aislados de individuos brasileños / Evaluation of changes of femoral torsion angle in brazilian individuals isolated femur

En corte axial del fémur, generalmente la orientación del eje del cuello femoral se ubica en diferente plano del eje de los cóndilos. La formación angular que el eje del cuello hace, en relación a la de los cóndilos, es conocida como ángulo de torsión femoral. Los autores realizaron mediciones de los ángulos de torsión (o antetorsión) en 56 fémures humanos adultos, de ambos sexos, siendo 26 derechos y 30 izquierdos. La literatura investigada presenta varios valores que varían de 8 a 25. Los valores obtenidos en las mediciones buscaron determinar el valor del ángulo de torsión en ese material y compararlo con los datos de la literatura. También se determinaron los valores que, permitieran conocer si el miembro inferior tenía rotación lateral o medial patológicas. Los valores obtenidos de los ángulos en las mediciones variaron entre 0 y 35. En el lado derecho, el promedio del ángulo de torsión fue de 20,8 y el del lado izquierdo de 14,86, lo que indica una mayor tendencia del lado derecho a tener anteversión.

In axial section of the fêmur, typically the axis direction of the femoral neck is located in different plane of the axis of the condoyle. The angle that the axis of the neck makes, when compared to the condoyle, is known as angle of femoral torsion. The authors carried out measurements of torsion angles or anteversion femurs in 56 human adults of both sexes, bein 26 right and 30 left. The literature shows several values ranging from 8-25 degrees. The values obtained in measurements seek to determine the value of the torsion angle in this material and compare with literature data. Also we determine values that, in tum, determine, from which, if the leg will feature lateral rotation, medial rotation or pathological. The values obtained in measurements of the angles between zero and thirty-five degrees. On the obtained in measurements of the angles zero and thirty-five degrees. On the right side, the average torsion angle was 20.8 and the left side of 14.86, indication a greater tendency to have the right anteversion.

relationship between normal intercondylar and intermaleolar distances and epidemiological factors in children 3-16 years of age

In evaluation of genu varum and genu valgum which are common in growth period of children, tibiofemoral [TF], intercondylar [1C] and intermaleolar [IM] distances are commonly measured. Normally, the legs of most newborns are slightly curved or have moderate curves medially when they are in standing position. During their growth, the extension of the lower limb changes. The aim of this study was to determine the relationship between normal intercondylar and intermaleoar distances and physiolgical factors among these subject groups. In this cross-sectional study a group of 850 children [452 females, 398 males] aged between 3 to 16 years old were studied. The samples were collected randomly from kindergartens, preschool care centers and schools in all 4 districts of Ahvaz, plus a few patients in Shafa hospital. The intercondylar and intermaleolar distance were examined in standing position, while the knee and hip joints were fully extended and the two medial maleolus or two medial condye of knees were in contact. In this study, descriptive statistics as well as t- and ANOVA tests were used for comparison. Pearson correlation for the survey were used data analysis by SPSS version 15. The mean of intercondylar distance among studied groups was 1.65 +/- 0.86: 1.5 in boys and 1.71 cm in girls respectively. The mean of intermaleolar distances was 2.31cm in males 2.05cm and in females 2.53 cm, the difference was significant [P<0.05]. There was a direct ralationship between intermaleolar distance and weight, height and length of leg, but relationship between Intercondylar distance and these factors is in reverse order

Repercussões do exercício físico durante a gestação e da desnutrição proteica durante a gestação e lactação sobre o desenvolvimento e crescimento do fêmur da prole / Effects of physical exercise during pregnancy and protein malnutrition during pregnancy and lactation on the development and growth of the offspring's femur

OBJETIVO: Avaliar os efeitos do treino físico imposto às ratas mães durante a gestação associado a uma dieta hipoproteica imposta durante os períodos gestacional e de lactação sobre o desenvolvimento e crescimento do fêmur da prole. MÉTODOS: Quarenta ratos machos Wistar, 90 dias de vida, foram divididos em quatro grupos: filhotes de mãe sedentária nutrida, filhotes de mãe sedentária desnutrida, filhotes de mãe treinada nutrida e filhotes de mãe treinada desnutrida, todos com n = 10. Treinamento físico em esteira durante 8 semanas, 5 semanas antes da concepção e 3 no período gestacional para as ratas mães dos grupos filhotes de mães treinada nutrida e treinada desnutrida. Indução da dieta hipoproteica às ratas mães durante a gestação e lactação para os grupos filhotes de mães sedentária desnutrida e treinada desnutrida. Após sacrifício, no 90º dia de vida, analisou-se peso, comprimento e conteúdo mineral ósseo do fêmur dos filhotes. RESULTADOS: Peso corporal, peso femoral e comprimento femoral diminuídos (p < 0,05) para os grupos filhotes de mães sedentária desnutrida e treinada desnutrida em relação aos grupos filhotes de mães sedentária nutrida e treinada nutrida, respectivamente. Não houve diferença no conteúdo mineral ósseo do fêmur em nenhum dos grupos. CONCLUSÃO: O treinamento físico leve em esteira durante a gestação não interfere no desenvolvimento e crescimento ósseo da prole. Entretanto, a desnutrição proteica durante esse período e durante a lactação promove prejuízos permanentes à estrutura óssea da prole.

OBJECTIVE: To evaluate the effects of physical training of mother rats during pregnancy associated with a low-protein diet offered during pregnancy and lactation on the development and growth of the femur of their offspring. METHODS: Forty 90-day old male Wistar rats were divided into four groups: pups of sedentary nourished mothers, pups of sedentary malnourished mothers, pups of trained nourished mothers, and pups of trained malnourished mothers; all groups included 10 rats. Physical training on a treadmill for 8 weeks, 5 weeks before conception and 3 weeks in the gestational period for mother rats of pups of trained nourished mothers and pups of trained malnourished mothers. Induction of low-protein diet to the mother rats during pregnancy and lactation for the groups of pups of sedentary malnourished mothers and trained malnourished mothers. After the pups were sacrificed, on the 90th day of life, we analyzed weight, length, and femoral bone mineral content. RESULTS: Decreased body weight, femur weight, and femur length (p < 0.05) were observed for the groups of pups of sedentary malnourished mothers and trained malnourished mothers in comparison with to the groups of pups of sedentary nourished mothers and trained nourished mothers, respectively. There was no difference in bone mineral content of the femur in either of the groups. CONCLUSION: Mild of physical training on the treadmill during pregnancy does not interfere with bone development and growth of the offspring. However, protein malnutrition during this period and during lactation promotes permanent damage to the bone structure of the offspring.

Hipercrescimento femoral no tratamento cirúrgico do quadril displásico inveterado / Femoral overgrowth following surgical treatment of long-established dysplasia of the hip

OBJETIVO: Medir radiograficamente através de escanometria o hipercrescimento femoral em pacientes portadores de Displasia do Desenvolvimento do Quadril inveterada tratados cirurgicamente com encurtamento femoral, redução cruenta e acetabuloplastia. MÉTODOS: Avaliamos 30 crianças (33 quadris) submetidas à redução cruenta pela técnica de Scaglietti e Calandriello, ostectomia para encurtamento femoral e acetabuloplastia de Salter. Haviam 29 do sexo feminino e 1 do sexo masculino, com idade média de 4 anos e 5 meses na ocasião da cirurgia. De acordo com a classificação de Zionts e MacEwen, 23 (69,6 por cento) quadris foram classificados como tipo III, 5 (15,2 por cento) como tipo I e 5 (15,2 por cento) como tipo II. O encurtamento femoral médio foi 45,12mm (variando de 30,00mm a 80,00mm). O tempo de seguimento médio foi de 10 anos e 2 meses. A discrepância femoral média mensurada nos escanogramas foi 13,48mm (variando de 0,00mm a 60,00mm) após acompanhamento mínimo de 2 anos e 3 meses. RESULTADOS: Todos os pacientes evoluíram com hipercrescimento sendo que em 18 (54,6 por cento) casos a anisomelia observada foi <30mm, 11 (33,3 por cento) alcançaram igualdade de comprimento e 4 (12,1 por cento) discrepância >30mm. CONCLUSÃO: Observamos diminuição significante na diferença entre os comprimentos femorais após tratamento cirúrgico comparando com as medidas obtidas durante o seguimento ambulatorial.

OBJECTIVE: To measure femoral overgrowth using radiographic scanning in patients with long-established Developmental Dysplasia of the Hip treated with femoral shaft shortening, open reduction and acetabuloplasty. METHODS: We studied 30 children (33 hips) submitted to surgical treatment including femoral shaft shortening, open reduction according to Scaglietti & Calandriello's procedure and Salter acetabuloplasty without preliminary traction. There were 29 females and 1 male, with mean age = 4 years and 5 months at the time of operation. According to Zionts & MacEwen's classification, 23 hips were classified as type III (69.6 percent), 5 (15.2 percent) as type I and 5 (15.2 percent) as type II. The average femoral shortening was 45.12 mm (range: 30.00 mm to 80.00 mm). The mean follow-up time was 10 years and 2 months (range: 2 years and 3 months to 18 years) and we noticed a mean femoral discrepancy of 13.48mm (range: 0.00 mm to 60.00 mm) using plain scanning images. RESULTS: All patients evolved to femoral overgrowth; in 18 cases (54.6 percent), the leg length discrepancy found was <30 mm, 11 (33.3 percent) showed no LLD, and 4 (12.1 percent) presented with a discrepancy >30mm. Conclusion: We noticed a significantly decreased discrepancy of femurs after surgical treatment when compared to the measurements obtained during outpatient follow-up.

Gadolinium-enhanced MR imaging of epiphyseal and metaphyseal marrow in normal piglets / 华中科技大学学报（医学）（英德文版）

The purpose of this study is to define the appearance of normal epiphyseal and metaphyseal marrow and normal changes of marrow due to fatty conversion on Gadolinium (Gd)-enhanced MR Imaging. Unenhanced and enhanced T1-weighted MR imaging were performed in proximal and distal femoral ends of 8 healthy piglets at the ages of 2, 4, 6 and 8 weeks, respectively. The changes with age in signal intensity and enhancement ratio of the epiphyseal and metaphyseal marrow with age were examined. The correlation of MRI characteristics with histological findings was studied. Our study showed that marrow of the metaphysis and of periphery of the 2nd ossification center were well vascularized hematopoietic marrow and had great enhancements. The enhancement ratio of metaphysis was greater than that of epiphyseal marrow and both enhancement ratios degraded gradually with age. The central regions of the epiphyseal ossification center and of the diaphysis were of fatty marrow and had little enhancement. It is concluded that on Gd-enhanced MR imaging the hematopoietic marrow of metaphysis and of periphery of the 2nd ossification center had greater enhancement than that of fatty marrow of central region of the 2nd ossification center. All of their enhancements decreased gradually with age.

MRI characteristics and transverse relaxation time measurements in normal growing cartilage / 华中科技大学学报（医学）（英德文版）

To determine the characteristics of magnetic resonance (MR) signals of normal growing cartilage and identify the difference in transverse relaxation times between physeal and epiphyseal cartilage in vivo. 24 distal femora of 12 two-week-old piglets were imaged on a 1.5 Tesla GE MR scanner. Comparison was made between signal intensity on MR images and the structure shown in corresponding histologic sections. T2 values were measured in eight piglets by means of multiecho spin-echo sequences. Our results showed that MR imaging delineated five regions between the secondary ossification center and the metaphysis, which histologically correspond to the zone of provisional calcification of the secondary ossification center, physis of the secondary ossification center, epiphyseal cartilage, physis and zone of provisional calcification. The T2 value in the physeal cartilage was much larger than that in the epiphyseal cartilage (P<0.05). It is concluded that MRI findings could differentiate the different regions of growing cartilage. T2 is longer in physeal than in epiphyseal cartilage, perhaps reflecting differences in water binding by proteoglycans.

Osteogénesis imperfecta. Experiencia del Hospital Shriners, Unidad Ciudad de México / Osteogenesis imperfecta. Experience in the Shriners Hospital from Mexico City

Desde su primera descripción en 1751, la osteogénesis imperfecta (OI) es una enfermedad de gran interés para el ortopedista; múltiples han sido los médicos e investigadores que han tratado de explicar la etiología, por lo que se han propuesto varias clasificaciones. De 1979 a 1993, se encontraron 57 expedientes de pacientes con OI en nuestro hospital, de los cuales sólo 39 se incluyeron en este estudio. La edad varió desde recién nacidos hasta 16 años, con un promedio de ocho años. Veinte eran del sexo masculino y 19 del femenino. Usando la clasificación de Seedorf encontramos que nueve pacientes eran del tipo I, 17 al tipo II y 3 del tipo III. La presencia de fracturas fue la manifestación clínica más frecuente, siendo el fémur el hueso predominante, con un promedio de 4.4 fracturas por paciente. Dentro del estudio encontramos que la OI tipo tarda gravis es la más frecuente; las deformidades óseas más frecuentes son en las extremidades inferiores: angulación anterolateral del fémur y tibia en sable. Es ideal la correción temprana de las angulaciones óseas, para permitir la deambulación temprana y mediante la ferulización interna prevenir la presentación de nuevas deformidades

Tratamiento de las lesiones traumáticas del extremo proximal del fémur en crecimiento / Treatment of traumatic injuries of the growning proximal femur

Este es un estudio prospectivo, longitudinal, descriptivo y observacional de las lesiones traúmaticas del extremo proximal del fémur en crecimiento. Se revisaron 22 pacientes, cuyas edades oscilaron desde recién nacido hasta 16 años; fueron del sexo masculino y 8 del sexo femenino, en el periodo de 1985 a octubre de 1997. El lado derecho es el más afectado, con un total de 14 pacientes. Se uso la clasificación de Delbet-Colonna, constituyéndose en cinco grupos. En el primer grupo, de la lesión fisaria cefálica, tuvimos tres pacientes: en el segundo grupo, de la lesión transcervical, siete casos; de la lesión basicervical fue el tercer grupo, con siete casos; del cuarto grupo de la lesión intertrocantérea, tres casos y en el último grupo, de la lesión subtrocantérea, dos pacientes. En todos los casos se obtuvo reducción anatómica de la lesión, consolidación de la fractura; y en el caso de los procedimientos quirúrgicos, no se presentaron infecciones. Un caso evolucionó al cierre de la fisis de la cabeza femoral y posteriormente necrosis avascular. Otro caso desarrolló coxa vara. Consideramos que la lesión traumática del extremo proximal del fémur en crecimiento debe ser cuidadosamente clasificada para brindar un óptimo tratamiento, utilizar en caso necesario, el material de osteosíntesis adecuado; llevar a la lesión a la reducción anatómica posible. Se debe revisar al paciente hasta que cese su crecimiento, para detectar futuras complicaciones

Medida ultra-sonográfica do diâmetro transverso do cerebelo fetal em gestaçöes normais / Ultrasound measurement of fetal transverse cerebellar diameter in normal pregnancy

Foram estudadas 535 gestantes atendidas na Clínica de Ultra-sonografia da Barra Ltda., Rio de Janeiro. As pacientes tinham data precisa da última menstruaçäo e näo apresentavam, por ocasiäo do exame, intercorrências clínicas e/ou obstétrica. As avaliaçöes ultra-sonográfics foram feitas do exame em pacientes com gestaçöes únicas, entre 15 e 40 semanas. Foi utilizado aparelho SSH-140 A (Toshiba Corporation) com transdutor convexo de 3,75 MHz, caliper multidirecional ajustado para velocidade assumida de ultra-som de 1540 metros/segundo. O diâmetro transverso do cerebelo foi aferido em milímetros pela técnica descrita por Goldstein e utilizado o diâmetro biparietal e comprimento do fêmur para a confirmaçäo da idade gestacional. Os dados foram submetidos a um programa de regressäo, sendo a equaçäo polinominal de terceiro grau, a que melhor se ajustou à nossa casuística. Os gráficos e tabelas foram construídos com limite superior e inferior no intervalo da confiança 95,44 por cento. Concluiu-se que a medida ultra-sonográfica do diâmetro transverso do cerebelo fetal correlaciona-se fortemente com a idade gestacional, sendo útil para a sua estimativa (r=0,98175, p<0,001)

Ensaio de flexäo em três pontos nas faces anterior e posterior de fêmures de ratas, em duas fases diferentes de crescimento / Flection trials in 3 points in anterior and posterior region of rats femurs, in 2 different phases of growth

Este estudo teve como objetivo analisar a comportamento mecânico em fêmures de ratas com 25 e 79 dias de vida, realizando ensaios de flexäo com cargas aplicadas nas faces côncava e convexa da diáfise deste osso. Os ensaios foram realizados em uma máquina universal de ensaios. Analisando os resultados dos parâmetros mecânicos, verificamos que foram significativamente maiores para os animais mais maduros em relaçäo às faces de aplicaçäo da força onde de maneira geral, näo houve diferença significativa fosse a força aplicada na concavidade ou na convexidade do osso

Distraçäo da placa epifisária: estudo experimental em coelhos / Distraction of the epiphyseal growth plate: experimental study in rabbits

Estudo experimental foi realizado na extremidade distal do fêmur em nove coelhos com doze semanas de vida, com o propósito de identificar o mecanismo básico envolvido na distraçao da fise, quando empregada taxa de distraçao de 1,0mm, numa única vez ao dia, e de avaliar a viabilidade do segmento alongado. Os animais foram divididos em três grupos, de acordo com a época em que forma mortos (final do alongamento, 30 e 60 dias após o alongamento). Os resultados radiográficos mostraram, em todos os espécimes, diástase da epífise da metáfise (GAP) entre o segundo e o quinto dias após o início do alongamento. Sessenta dias após o período distracional, ficou evidenciado radiograficamente que todo o segmento alongado estava ossificado. Os resultados histológicos obtidos mostraram que o mecanismo básico envolvido em alongamento foi epifisiólise distracional, produzida por fratura através da zona hipertrófica entre a camada degenerativa e a de calcificaçao provisória da placa epifisária. Após 60 dias do término do alongamento, a placa epifisária havia retornado à espessura normal e sua morfologia apresentava características semelhantes às da fise normal. O segmento alongado estava ocupado por tecido ósseo. A distraçao de 1,0mm, numa única vez ao dia (espifiólise distracional) da placa epifisária, provou ser eficaz na obtençao de alongamento ósseo em coelhos, pois levou à formaçao de um osso novo com características morfológicas e funcionalmente semelhantes ao osso normal. Portanto, esse método de alongamento é mais uma opçao para correçao de discrepâncias entre os membros.

Effect of gamma-irradiation on the development of the femur of the chick embryo

In this work 90 fresh fertile Dokki-4 eggs were used. The eggs were divided into two groups. The first group [30 eggs] was regarded as control. The second group [60 eggs] was irradiated with a single dose [50 rads] of gamma rays on the 48 hours of incubation. The embryos were extracted on the 14th and 19th days of incubation, decalcified and fixed in Bouin's fluid for 7 days. Paraffin sections were prepared in the usual manner and stained with Hx and E. The femur of 14 and 19 days irradiated chick embryos showed evidence of retardation of growth, as diminution of the length and deformity of the shape of the femur. Thin periosteum with interrupted bone trabeculae were present among the changes seen in the femur. Also, wideness of the bone marrow cavity with necrosis of its content were found. The osteoblasts and the osteocytes appeared small in size with decrease in their number